calcein am pi staining Search Results


calcein-am/pi double stain kit  (Dojindo Labs)
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Dojindo Labs calcein-am/pi double stain kit
Calcein Am/Pi Double Stain Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein-am/pi live/dead cell double staining kit  (Beijing Solarbio Science)
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Beijing Solarbio Science calcein-am/pi live/dead cell double staining kit
Calcein Am/Pi Live/Dead Cell Double Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein-am/pi double stain kit  (Yeasen Biotechnology)
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Yeasen Biotechnology calcein-am/pi double stain kit
Calcein Am/Pi Double Stain Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein am/pi double staining  (Keygen Biotech)
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Keygen Biotech calcein am/pi double staining
Calcein Am/Pi Double Staining, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein-am/propidium iodide am/pi  (Keygen Biotech)
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Keygen Biotech calcein-am/propidium iodide am/pi
Calcein Am/Propidium Iodide Am/Pi, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein am/pi double staining kit e-ck-a354  (Elabscience Biotechnology)
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Elabscience Biotechnology calcein am/pi double staining kit e-ck-a354
Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, <t>Calcein</t> AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Calcein Am/Pi Double Staining Kit E Ck A354, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein am and pi double staining  (Elabscience Biotechnology)
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Elabscience Biotechnology calcein am and pi double staining
Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, <t>Calcein</t> AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Calcein Am And Pi Double Staining, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell stain double-staining kit containing calcein–acetoxymethyl (am) propidium iodide (pi  (Dojindo Labs)
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Dojindo Labs cell stain double-staining kit containing calcein–acetoxymethyl (am) propidium iodide (pi
Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, <t>Calcein</t> AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Cell Stain Double Staining Kit Containing Calcein–Acetoxymethyl (Am) Propidium Iodide (Pi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein-am/pi double staining kit hb210602  (Yeasen Biotechnology)
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Yeasen Biotechnology calcein-am/pi double staining kit hb210602
Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, <t>Calcein</t> AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Calcein Am/Pi Double Staining Kit Hb210602, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein-am/pi double stain kit cell staining agent  (Yesen Biotech)
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Yesen Biotech calcein-am/pi double stain kit cell staining agent
Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, <t>Calcein</t> AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Calcein Am/Pi Double Stain Kit Cell Staining Agent, supplied by Yesen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence stain calcein-am/pi  (Dojindo Labs)
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Dojindo Labs fluorescence stain calcein-am/pi
Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, <t>Calcein</t> AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Fluorescence Stain Calcein Am/Pi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calcein-am/propidium iodide (pi) staining method  (Promega)
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Promega calcein-am/propidium iodide (pi) staining method
(A) 4T1 cells were treated with BSA Cu(DDC)2 MONs for 18 h. (B) MDA-MB-231 were treated with BSA Cu(DDC)2 MONs for 48 h. Then, cells were stained with <t>Calcein-AM</t> (green) and PI (red) at the end of drug treatment.
Calcein Am/Propidium Iodide (Pi) Staining Method, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, Calcein AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: International Journal of Biological Sciences

Article Title: Ferroptosis inhibitor alleviates sorafenib-induced cardiotoxicity by attenuating KLF11-mediated FSP1-dependent ferroptosis

doi: 10.7150/ijbs.86479

Figure Lengend Snippet: Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, Calcein AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Live cell staining was carried out using Calcein AM/PI Double Staining Kit (E-CK-A354, Elabscience).

Techniques: Western Blot, Negative Control, Expressing, Staining, Transmission Assay, Microscopy

(A) 4T1 cells were treated with BSA Cu(DDC)2 MONs for 18 h. (B) MDA-MB-231 were treated with BSA Cu(DDC)2 MONs for 48 h. Then, cells were stained with Calcein-AM (green) and PI (red) at the end of drug treatment.

Journal: Applied materials today

Article Title: Biomimetic metal-organic nanoparticles prepared with a 3D-printed microfluidic device as a novel formulation for disulfiram-based therapy against breast cancer

doi: 10.1016/j.apmt.2019.100492

Figure Lengend Snippet: (A) 4T1 cells were treated with BSA Cu(DDC)2 MONs for 18 h. (B) MDA-MB-231 were treated with BSA Cu(DDC)2 MONs for 48 h. Then, cells were stained with Calcein-AM (green) and PI (red) at the end of drug treatment.

Article Snippet: The cytotoxicity was determined with the Calcein-AM/Propidium Iodide (PI) staining method as well as CellTiter-Blue reagents (Promega).

Techniques: Staining

Tumor spheroids were treated with CuCl2 (1μM), DDC-Na (2μM), and BSA Cu(DDC)2 MONs (1μM) for 72 h. At the end of treatment, tumor spheroids were analyzed with (A) Calcein-AM/PI staining, and (B) CellTiter-Blue Cell Viability Assay. Results are the mean ± SD (n = 3). ***, P < 0.001, and **, P < 0.01.

Journal: Applied materials today

Article Title: Biomimetic metal-organic nanoparticles prepared with a 3D-printed microfluidic device as a novel formulation for disulfiram-based therapy against breast cancer

doi: 10.1016/j.apmt.2019.100492

Figure Lengend Snippet: Tumor spheroids were treated with CuCl2 (1μM), DDC-Na (2μM), and BSA Cu(DDC)2 MONs (1μM) for 72 h. At the end of treatment, tumor spheroids were analyzed with (A) Calcein-AM/PI staining, and (B) CellTiter-Blue Cell Viability Assay. Results are the mean ± SD (n = 3). ***, P < 0.001, and **, P < 0.01.

Article Snippet: The cytotoxicity was determined with the Calcein-AM/Propidium Iodide (PI) staining method as well as CellTiter-Blue reagents (Promega).

Techniques: Staining, Viability Assay